TP Microscopy and Image Analysis

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Technology Platform Microscopy and Image Analysis at the Heinrich Pette Institute, Leibniz Institute for Experimental Virology

Last updated: 19 May 2017

This Entity is a Research Group and Facility: Usage Guidelines
Number of facility users per year: 50


The subjects and methods of the technology platform strictly adhere to the institute’s development and research goals in both program areas and consolidate its core competencies in methods and technology. The microscopy and image analysis technology platform functions as a research based service unit associated with the department for structural biology of viruses. Using the latest technology it enables holistic investigations of infection processes with sophisticated imaging technology across a broad range of resolution and complexity levels. A research based infrastructure design also ensures that technologies are continuously developed at a high standard and allows the HPI to expand its involvement in research based services. The technology platform incorporates the Nikon Center of Excellence for Integrative Microscopy.


Please visit the website: http://www.hpi-hamburg.de/de/forschung/technologien/mikroskopie-bildanalyse/

Address

Technology Platform Microscopy and Image Analysis at the Heinrich Pette Institute, Leibniz Institute for Experimental Virology
Martinistr. 52
20251 Hamburg


Head of Group

Dr. Rudolph Reimer
Position: Head of Imaging Facility
rudolph.reimer@hpi.uni-hamburg.de
Office: +49-40-48051-254

Techniques

  • Laser Scanning Confocal (LSM) (+) ,
  • Spinning Disc Confocal (+) ,
  • Total Internal Reflection Fluorescence (TIRF) (+) ,
  • Deconvolution widefield microscopy (+) ,
  • Single Plane Illumination Microscopy (SPIM) (+) ,
  • Single Molecule Imaging Techniques (+) ,
  • Stochastic Optical Reconstruction Microscopy (STORM) (+) ,
  • Photoactivated Localization Microscopy (PALM) (+) ,
  • Functional Imaging of Living Cells (+) ,
  • Serial Block-Face Electron Microscopy (SBFSEM) (+) ,
  • Transmission Electron Microscopy (TEM) (+) ,
  • High Speed Confocal (more than 20 Hz full frame) (+) ,
  • Image Analysis (+) ,
  • Scanning Transmission Electron Microscopy (STEM) (+) ,
  • Scanning Electron Microscopy (SEM) (+) ,
  • Electron Tomography (ET) (+) ,
  • Correlative Light and Electron Microscopy (CLEM) (+) ,
  • Ultramicrotomy (+) ,
  • Immuno-Electron Microscopy (+) ,
  • High-Pressure Freezing (HPF) (+) ,
  • Rapid Plunge Freezing (Plunge) (+) ,
  • Freeze substitution (+) ,
  • Freeze-fracture EM (+) ,
  • Cryo-Ultramicrotomy (Tokuyasu technique) (+) ,
  • Immunohistology / -cytochemistry (+) ,
  • Histological staining (+) ,
  • Negative staining of particles (+) ,
  • Embedding (Epon/Paraffin/PLT) (+) ,
  • Microtomy (Cryostat/Vibratome/Paraffin/Resin) (+)

Environmental Scanning Electron Microscopy (ESEM)

Group Members

 TitlePositionContact FormPhone
Carola SchneiderTechnician+49-40-48051-250
Dennis EggertDr.Visiting Scientist+49-40-48051-294
Gundula Pilnitz-StolzeTechnician+49-40-48051-236
Jens BosseDr.Head of Imaging Facility+49-40-48051-273
Rudolph ReimerDr.Head of Imaging Facility+49-40-48051-254