Simons Lab

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Simons Lab - Institute of Neuronal Cell Biology - TUM

Last updated: 18 March 2018

This Entity is a Microscopy Research Group:


We are part of the Institute of Neuronal Cell Biology at the School of Medicine of the Technical University of Munich (TUM), and are affiliated with the German Center for Neurodegenerative Diseases (DZNE) and the Excellence Cluster SyNergy.


Please visit the website: http://agsimons.weebly.com/

Address

Simons Lab - Institute of Neuronal Cell Biology - TUM
Feodor-Lynen-Straße 17
81377 Munich


Head of Group

Prof. Dr. Mikael Simons
Position: Head of Research Group (PI)

Techniques

  • Laser Scanning Confocal (LSM) (+) ,
  • Spinning Disc Confocal (+) ,
  • Deconvolution widefield microscopy (+) ,
  • Functional Imaging of Living Cells (+) ,
  • Image Analysis (+) ,
  • High-Pressure Freezing (HPF) (+)

Microscopes


Leica SP8 CLSM with HyVolution2 and Live Data modules, Galvo stage, 2PMTs, 2HyDs and 1TL-PMT. Laser lines: 405nm, 448nm, 488nm, 514nm and 552nm. Equipped with live imaging setup. Optimized for long term zebrafish live imaging.

Leica SP5 CLSM with AOBS, Galvo stage, 5PMTs and 1TL-PMT. Laser lines: 405nm, 458nm, 476nm, 488nm, 496nm, 514nm, 561nm, 594nm, 633nm.

Nikon C2+ Confocal. With temperature controller and CO2 mixer for live imaging and 3PMTs. Laser lines: 405nm, 488nm, 561nm and 640nm. ​Objectives: 10x and 20x Air, 60x Oil immersion and 100x Oil /TIRF.

Leica DMI6000B widefield: With temperature controller and CO2 mixer for live imaging and also with Adaptive Focus Control (AFC). Capable for the following contrast methods: Brightfield, Phase contrast, Polarization contrast, Fluorescence (DAPI, eGFP, Cy3, Cy5). Objectives: 10x, 20x and 40x Air, 63x Oil immersion

Image Analysis Workstations


Dell precision T7910 with Intel(R) Xeon(R) CPU E5-2697 v3 @2.6GHz and 128GB RAM, nVidia Quadro M5000

Dell precision T7910 with Intel(R) Xeon(R) CPU E5-2697 v3 @2.6GHz and 64GB RAM, nVidia Quadro K2200

HP Z640 Workstation with Intel(R) Xeon(R) CPU E5-2650 v3 and 192GB RAM, nVidia Quadro M6000 12GB vRAM

Software


Imaris - Bitplane

Huygens Deconvolution - SVI

ImageJ/FIJI

Cell Profiler

Icy

Group Members

 TitlePositionContact FormPhone
Ioannis AlexopoulosDr.Core Facility Staff