Multiphoton Microscope Core Facility LMU

From GermanBioImaging
Jump to: navigation, search

at the Walter-Brendel-Zentrum für Experimentelle Medizin Ludwig-Maximilians-Universität München. The major aim of this central bio-imaging platform (infrastructure core facility) is to provide access to state-of-the-art two- and multiphoton in vivo imaging microscopy (MP-IVM) to the members of the Collaborative Research Centre 914 (SFB 914).


Loading map...

Multiphoton Microscope Core Facility, Walter-Brendel-Zentrum, LMU

Last updated: 12 October 2016

This Entity is a Canonical Facility: Usage Guidelines
Number of facility users per year: 0


We provide professional advice and assistance in planning and setting up microscopy experiments, sample preparation and imaging as well as in post-imaging reconstruction and analysis.


Please visit the website: http://www.sfb914.med.uni-muenchen.de/imaging_platforms/z01_mp-ivm/index.html

Address

Multiphoton Microscope Core Facility, Walter-Brendel-Zentrum, LMU
Marchioninistr. 27
81377 München


Head of Group

Dr. Hellen Ishikawa-Ankerhold
Position: Head of Imaging Facility
Hellen.Ishikawa-Ankerhold@med.uni-muenchen.de
Office: +49-89-2180-76581

Techniques

  • Laser Scanning Confocal (LSM) (+) ,
  • Spinning Disc Confocal (+) ,
  • 2-photon Scanning Confocal (2-P) (+) ,
  • Image Analysis (+) ,
  • Immunohistology / -cytochemistry (+) ,
  • Histological staining (+) ,
  • Microtomy (Cryostat/Vibratome/Paraffin/Resin) (+)

We provide animal models allowing MP-IVM of immune cells in several mouse tissues and organs, including bone marrow, lymph node, arterial wall, spleen, liver, gastric mucosa, ear, placenta, Yolk sac; Embrionic liver and cremaster muscle. We use transgenic report mice expressing fluorescent proteins in distinct cell lineages, including hematopoietic progenitor cells, monocytes, neutrophils, dendritic cells, or T cells. Imaging is performed under both non-infectious and infectious S2 safety conditions.

We provide professional advice and assistance in planning and setting up microscopy experiments, sample preparation and imaging as well as in post-imaging reconstruction and analysis using Volocity™, ImageJ™, ImSpector™ and Imaris software.

Group Members

 TitlePositionContact FormPhone
Hellen Ishikawa-AnkerholdDr.Head of Imaging Facility+49-89-2180-76581